Presence of 2-methylthioribosyl-trans-zeatin in Azotobacter vinelandii tRNA.
نویسندگان
چکیده
Hydroxylated cytokinin, 2-methylthio-N6-(4-hydroxy-3-methylbut-2-enyl) adenosine, was found in the tRNA of Azotobacter vinelandii. This cytokinin had the trans configuration, unlike the cis configuration reported for that from other bacteria. Culture-condition-dependent changes in the content of this thiocytokinin and a few other thionucleosides in the tRNA of this bacterium have been observed.
منابع مشابه
Cytokinins in Azotobacter vinelandii Culture Medium.
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. T...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 162 2 شماره
صفحات -
تاریخ انتشار 1985